# Data from Leob, et al. Complete mutagenesis of the HIV-1 protease. 
# Nature 340:397-400.  Please reference this article if you publish 
# using this data.
#
# + means substitution does not alter phenotype
# - means substitution affects protein
# +- and -+ do not affect protein as severely as "-"
# order of severity : - > -+ > +- > +
#
# Questions about the format of this dataset?  Email sift@fhcrc.org
# 
P1L +
P1S +-
P1H +
Q2E +
I3L +
I3T +-
I3N +
I3S -
T4S +
T4R +-
L5V -
L5H -
W6G +
W6C +
W6L +
Q7H +
Q7P - 
Q7L -
R8G -
R8L -
R8Q +
P9S +
P9T -
P9H -
P9R -
L10I +
L10V +
L10H +-
L10R +-
V11I +
V11A +-
V11G -
T12S +
T12I +
I13V +
I13L +-
I13M +-
K14T +
K14M +
K14N +
K14E +-
K14Q +
I15V +
I15T -
I15R -
G16V +-
G16W +-
G17W +-
G17E +
Q18L +
Q18H +
Q18R +
L19T +
K20M +
K20Q +-
E21Q +
E21V +
E21K +
A22G +-
A22T -
A22D -
L23V +-
L23P -
L23Q -
L23R -
L24M +-
L24V +-
L24S -
L24F -
D25A -
D25Y -
D25H -
T26A -
T26I -
T26K -
T26R - 
G27A -
G27P -
G27I -
G27V -
G27L -
G27R -
G27D -
A28G -
A28S -
A28T -
A28E -
D29E -
D29N -
D29A -
D29G -
D29V -
D30E +
D30N +-
D30Y +-
T31S +-
T31A -
T31I -
T31R -
V32L +
V32E -
L33V +
L33A -
L33G -
L33S -
L33I +
L33T -
L33N -
L33D -
L33Y -
L33E -
E34A +
E35S +
E35A +-
E35L +-
E35K +-
E35M +-
E35G +-
M36I +
M36R -
S37G +
S37R +
L38V +-
L38F +-
P39R +-
P39A +-
P39T +
P39L +-
G40E -
G40R -
R41K +
R41I +
W42G +
W42L -
W42R -
K43N +
K43Q +
P44T +
P44R +
K45R +
K45T +
K45Q +
M46L +
I47L -
I47M -
I47S -
I47T -
I47D -
I47E -
I47R -
G48S +
G48T +-
G48I +-
G48E +-
G48H +
G49V -
G49R -
I50L +
I50M +-
I50T -
I50N -
I50R -
G51A +
G51K -
G51V -
G51C -
G52A -
G52S -
G52V -
G52D -
F53Y +
F53I +
F53L +
F53V +
I54L +
I54S +-
I54T +-
I54F +-
K55R +
K55T +
K55I +
K55N +
K55Q +
V56I -
V56L -
V56G -
R57K +
R57G -
R57S -
R57T -
R57I -
Q58E +
Q58P -
Q58K +-
Q58H +-
Y59D -
Y59C -
D60E +
D60V +-
Q61P +-
Q61L +-
Q61R +
I62L +
L63I +
L63V +
L63P +
L63R +
I64L +
I64T +-
I64R -
E65V -
E65K -
I66L +
I66M +-
I66V +
I66S -
I66T -
I66F +
C67G +
C67F +
C67W +-
C67Y +
G68V -
G68E -
H69R +
H69L +
H69Y +
H69D -
H69N +-
H69Q +
K70T +
K70I +
K70N +
A71P -
A71L +
A71S +-
I72T +
I72L +
I72V +
G73R -
G73Q -
G73T +-
G73C +
G73V +-
G73A +-
G73K -
G73P -
T74S +
T74I -
V75E -
V75G -
L76I -
V77I +
V77G -
V77E -
G78A -
G78V -
G78E -
P79A +
P79S +
P79T +-
P79L -
T80P -
P81A -
P81T -
P81H -
P81R -
V82I +
V82L +
V82A +-
V82G -
V82T +
V82F +-
V82D -
N83D -
N83K -
N83I -
N83T -
I84L +-
I84M -
I84K -
I85L +-
I85S -
I85F -
I85N -
G86S -
G86I -
G86E -
G86N -
G86Q -
G86R -
G86K -
R87S -
N88A +-
L89V +
L90V -
L90S -
L90F -
L90W -
T91A +
T91P -
T91N +
Q92P -
Q92L +
Q92H +-
I93M +
I93V +
I93T +-
I93F +
I93N -
G94A -
G94V -
G94R +-
C95G -
C95S -
C95F +-
C95Y -
C95R -
T96P -
T96S +-
T96R +-
L97S -
L97F -
N98D +-
N98S +
N98I +-
N98Y +-
F99A -
F99P -
F99S -
F99L +
F99D -
F99K -
F99R -
F99Y +-
F99E -